Fig. 6.

Recruitment of ERα and SRC3 (AIB1) at PS2 proximal promoter region containing estrogen responsive element (ERE) in MCF7:WS8 and MCF7:5C cells. (A) Depiction of PS2 proximal promoter region and the ERE region relative to transcription start site (TSS). (B) MCF7:WS8 cells treated for 45 minutes with E₂ (1 nM), 3OHTPE (1 µM), EtOX (1 µM), bisphenol (1 µM), and 4OHT (1 µM), and ChIP assay was performed as described in Materials and Methods. (C) MCF7:5C cells were treated as mentioned above, and ChIP assay was performed under identical conditions. Data are represented as percent input of the starting chromatin used for the ChIP. Veh, vehicle.