Figure 4.

Penicillin-exposure does not reduce genomic DNA or pre-16S rRNA accumulation. Total DNA and RNA from 1X antibiotic-exposed, infected cells was subjected to semi-quantitative PCR (or RT-PCR) using primers specific for human GAPDH (DNA), chlamydial 16S rRNA (DNA), and chlamydial unprocessed 16S rRNA transcripts (RNA). (A) Representative PCR gel images. Amplification of + control DNA dilutions are shown to the left. (B) Plots of chlamydial genomic DNA amplimer quantity normalized to host genomes (left) and pre-ribosomal RNA-specific amplimer quantity normalized to chlamydial genomes (right). The average of three independent biologic replicates from one of three independent experiments ± s.e.m. is shown. None of the experimental groups were significantly different from the diluent-exposed, infected control at P ≤ 0.05.