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PCR conditions for each locus.
| atpB | ef-2 | glnA | ppsA | rpoB | |
|---|---|---|---|---|---|
| Water (μl) | 11.6 | 8.2 | 11.8 | 7.9 | 11.9 |
| 5× phire reaction buffer (μl) | 4.0 | 4.0 | 4.0 | 4.0 | 4.0 |
| DMSO (μl) | 0.6 | 0 | 0.4 | 0.6 | 0.6 |
| Acetamide (25%, μl) | 0 | 4.0 | 0 | 4.0 | 0 |
| dNTP mix (10 mM, μl) | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 |
| Forward primer (10 mM, μl) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Reverse primer (10 mM, μl) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Phire II DNA polymerase (μl) | 0.4 | 0.4 | 0.4 | 0.4 | 0.4 |
| Template DNA (20 ng/μl, μl) | 1.0 | 1.0 | 1.0 | 0.7 | 0.7 |
| Annealing temperature (°C) | 60.0 | 61.0 | 69.6 | 66.0 | 63.7 |
