Table 1.
Subunit composition of dimers, monomers and subassembled ATP synthase complexes determined by mass spectrometry
| Subunit | Mass (da) | PLGS score |
Peptides |
Coverage (%) |
Incidence of detection (/5) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| L/L |
L/L, cre |
|||||||||||||
| L/L | L/L, cre | L/L | L/L, cre | L/L | L/L, cre | V2 | V1 | V2 | V1 | subVa | subVb | F1 | ||
| α | 59 715 | 45 982 | 46 238 | 59 | 51 | 63 | 58 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| β | 56 265 | 31 324 | 32 068 | 38 | 32 | 75 | 56 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| γ | 16 751 | 8476 | 9171 | 14 | 10 | 36 | 34 | 5 | 5 | 5 | 5 | 5 | 2 | 4 |
| δ | 32 750 | 11 492 | 11 412 | 4 | 5 | 26 | 38 | 4 | 3 | 3 | 5 | 3 | 2 | 0 |
| ɛ | 5834 | 19 006 | 21 785 | 3 | 3 | 37 | 30 | 3 | 5 | 3 | 5 | 4 | 3 | 0 |
| OSCP | 23 348 | 14 405 | 20 730 | 18 | 13 | 68 | 60 | 5 | 5 | 5 | 5 | 5 | 5 | 3 |
| b | 28 930 | 3914 | 2688 | 9 | 7 | 33 | 24 | 5 | 5 | 4 | 5 | 4 | 1 | 0 |
| e | 8230 | 15 448 | 3020 | 5 | 6 | 47 | 53 | 5 | 5 | 5 | 5 | 3 | 2 | 4 |
| f | 8999 | 3516 | 4051 | 3 | 3 | 26 | 21 | 5 | 5 | 4 | 5 | 5 | 2 | 2 |
| g | 11 417 | 12 753 | 10 165 | 7 | 5 | 52 | 41 | 4 | 5 | 4 | 5 | 5 | 0 | 2 |
| ATP8 | 7761 | 6763 | 8802 | 3 | 2 | 28 | 24 | 5 | 5 | 5 | 4 | 4 | 0 | 0 |
The ATP synthase dimer (V2), monomer (V1), subassembled complexes (subVa and subVb) and F1 subunit composition in heart mitochondria from Lrpprc knockout and control mice at the age of 12 weeks (n = 5). The average PLGS score, number of peptides and coverage were calculated from values obtained in ATP synthase dimer (V2) and monomers (V1). The PLGS score was calculated by the Protein Lynx Global Server (PLGS 2.2.5) software and is a statistical measure of accuracy of assignation. A higher score implies greater confidence in protein identity. The incidence of detection in five replicates is presented in the right panel.