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. 2013 Dec 23;23(10):2629–2638. doi: 10.1093/hmg/ddt658

Figure 4.

Figure 4.

Detection of markers of the ISR in G85R and G85R/GADD34+/ΔC mice. (A) Representative western blots from differently aged G85R, G85R/GADD34+/ΔC, GADD34+/ΔC and control non-transgenic littermate mice immunostained with antibodies to phosphorylated (p)-eIF2α, ATF4 and CHOP. Anti-β tubulin antibody was used as a loading control. ‘Pre’ refers to prior to disease onset, ‘Wk’ refers to the early phase of disease, while ‘End’ refers to end stage; the approximate ages of the mice are provided. (B) Bar diagrams showing quantitation of UPR markers from western blots of homogenates of the lumbar spinal cord from G85R (n = 4) and G85R/GADD34+/ΔC (n = 4) mice at end stage of disease. The mean ± standard deviation is shown. The value of the specific ISR marker detected in G85R mice was arbitrarily set as 1. * P < 0.05, ** P < 0.001.