Figure 1. The axonal network of medulla dorsal cluster neurons (DCNs) in the adult central nervous system of Drosophila.
(A) Dorsal cluster neurons, labeled with lacZ (red) using the atoGal4-14a driver, with its dendritic and axonal projections in the optic lobes of the CNS. Using the FLP-out system, an individual neuron is labeled with mCD8-GFP (green) within the background of the entire cluster. AtoGal4-14a is used in all the following experiments except when stated otherwise. (B) DCN axons, labeled with mCD8-GFP, form a stereotypic pattern of axonal branches within the medulla (Me) of the adult optic lobe. (C) Using the FLP-out system the axon and branches of an individual neuron are labeled with mCD8-GFP (green) within the background of the entire cluster labeled with lacZ (red). (D) False color labeling of one Me DCN axon with its main shaft (green) and branches (magenta) using a tracer tool. The scale bars represent 100 µm in (A) and 20 µm in (B–D).