Fig. 3.

Repair of a dL lesion in the absence (▴) of 8 oxodGuo (Go) and in the presence of 8-oxodGuo (Go) in position −5 (■), −1 (•), +1 (○) and +5 (□). (A) APE1 activity as measured by cleavage of the intact strand. Reaction contains APE1 only. (B) Polymerase β activity as measured by incorporation of bases. Reaction contains APE1 and polymerase β. (C) FEN1 activity as measured by removal of the DNA flap. Reaction contains APE1, polymerase β and FEN1. (D) Ligase 1 activity as measured by restitution of the intact strand. Reaction contains APE1, polymerase β, FEN1 and ligase1. Error bars show standard error of the mean from three experiments.