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. Author manuscript; available in PMC: 2014 Sep 25.
Published in final edited form as: Nat Commun. 2014 Mar 25;5:3496. doi: 10.1038/ncomms4496

Figure 10. The amount of sarkosyl-insoluble human tau is inversely proportional to that of human NDP52.

Figure 10

(a) Homogenates from cerebral cortical tissue of a diagnosed AD case were used for the co-immunoprecipitation of human NDP52 (hNDP52) using a Tau5, 12E8 or PHF1 antibody. (b) Homogenates used for co-immunoprecipitation in (a) were analyzed by immunoblotting using anti-NDP52, Tau5, 12E8 or PHF1 antibody. (c) Cerebral cortical tissues from diagnosed AD cases were separated into sarkosyl-soluble and insoluble fractions. The levels of total tau and tau phosphorylated at Ser262/Ser356 and Ser396/Ser404 in the sarkosyl-insoluble fractions were analyzed by immunoblotting using a 12E8, PHF1 and polyclonal to total tau (Tau) antibody, respectively. The levels of hNDP52, p62/SQSTM1 and LC3 were examined by immunoblotting. The relative molecular masses (kD) are indicated to the left of each blot. (d, e, f) The regression graphs show the relative amount of tau phosphorylated at Ser262/Ser356 (12E8) (d), at Ser396/Ser404 (PHF1) (e) or total tau (f) contrast to hNDP52 present in the sarkosyl-insoluble fractions.