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. 2014 Apr 11;9:1815–1822. doi: 10.2147/IJN.S58269

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© 2014 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Culture and characterization of bone marrow-derived EPCs.

Notes: Bone marrow-derived mononuclear cells (MNCs) demonstrated a spindle-shaped morphology (A) after in vitro expansion (×200). Cells cultured in endothelium culture medium are positive for Dil-ac-LDL (B) and UEA1 (C) uptake. Double positive cells for Dil-ac-LDL/UEA1 uptake demonstrate yellow color (D).

Abbreviations: Dil-Ac-LDL, 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate labeled acetylated low-density lipoprotein; EPC, epithelial progenitor cell; UEA, ulex europaeus agglutinin.