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. Author manuscript; available in PMC: 2015 Feb 15.
Published in final edited form as: Cancer Res. 2013 Dec 26;74(4):1250–1260. doi: 10.1158/0008-5472.CAN-13-1778

Figure 2. Stem-like mammary epithelial cells have microtentacle-promoting cytoskeletal alterations.

Figure 2

A) Representative western blot analysis of HMLE subpopulations shows that stem-like HMLEs have increased vimentin and detyrosinated tubulin (Glu) whereas total α-tubulin and GAPDH are unchanged (cropped). B) Attached non-stem-like HMLE cells show weak, diffuse staining for Glu-tubulin (b) and a loss of vimentin protein and organization (c), whereas stem-like HMLE cells display bundling of filamentous Glu-tubulin (f) and a robust network of vimentin filaments (g). Hoechst was used to visualize the nuclei (d, h). C) Suspended immunofluorescence where detached HMLE subpopulations were spun down onto glass coverslips and fixed. Immunostaining reveals the presence of Glu-tubulin (f) and vimentin (g) in McTN protrusions (white arrows) in the stem-like HMLE subpopulation but not in the non-stem-like subpopulation (b,c). Hoescht was used to visualize the nuclei (a,e).