Fig. 3.

Frequency and function of CD4⁺CD25⁺ Tregs in transgenic mice. Percentages of CD4⁺CD25⁺ Tregs in CD4⁺ T cells are shown for TG and LMC mice for spleens (A) and pancreatic lymphnodes (PLN) (B). Each point represents a single mouse and means are shown within each group. (C) In vitro suppression assay: CD4⁺CD25⁻ Tresps are co-cultured with CD4⁺CD25⁺ Tregs purified from spleens using different Treg/Tresp ratios. Criss-cross suppression assays are also performed by using Tregs from TG mice co-cultured with Tresp from LMC, and Treg from LMC co-cultured with Tresp from TG mice. Results are expressed as the mean of triplicate culture.