B-galactosidase stained sections of control sympathetic trunk ganglia (A) and magnification (B) at E16.5. Dbh ISH of H2CKO sympathetic trunk ganglia (C), and magnification (D) at E16.5. Arrows in (B, D) indicate ganglia. B-galactosidase stained sections of control (E), and H2CKO (F) adrenal medulla at E16.5. Hand2 ISH of control (G) and H2CKO (H) adrenal medulla at P3. Dbh ISH of control (I) and H2CKO (J) adrenal medulla at P3. qRT-PCR of Hand2, Dbh, Th, and Pnmt in isolated P3 adrenal glands (K). Asterisks in (H, J) indicate reduction of Hand2 and Dbh respectively. Asterisks in (K) indicate p-values less than 0.05.