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. Author manuscript; available in PMC: 2014 Oct 15.
Published in final edited form as: Cancer Res. 2014 Mar 31;74(8):2246–2257. doi: 10.1158/0008-5472.CAN-13-2131

Figure 2.

Figure 2

HOIP is not required for DNA damage checkpoint activation. A, HEK293 cells were transfected with siCON or siHOIP and treated with 5 μM cisplatin for the indicated times. Cell lysates were analyzed by immunoblotting with the antibodies indicated. B, siCON or siHOIP transfected HEK293 cells were treated with 8 μM cisplatin for 2 hours before being allowed to recover for the times indicated. Cells were then fixed and stained with DAPI to visualize nuclei and γ-H2AX antibody to visualize γ-H2AX foci. C, quantitation of the data in (B): cells with more than 6 foci were classed as γ-H2AX positive. Data in (C) is presented as mean ± SEM from three independent experiments.