Table 2. McDonald-Kreitman test for selection in pvmsp-1.

McDonald-Kreitman Comparisons
	42 kDa region		42 kDa intervening region
	Synonymous	Non-synonymous	Synonymous	Non-synonymous
Fixed	96	93	32	28
Polymorphic	34	39	10	31
	p = 0.681		p = 0.00427

Evidence for long-term selective pressure on the pvmsp-1 42 kDa region and the 42 kDa intervening region was assessed with the McDonald-Kreitman test, using P. knowlesi msp1 as the outgroup comparator. A Fisher's exact test (two tailed) was used to determine significance.