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. 2014 Apr 17;8(4):e2806. doi: 10.1371/journal.pntd.0002806

Figure 4. Reproducibility of spliced leader priming and amplification.

Figure 4

Transcriptomes are represented as in Figure 1; results for individual experiments are compared. A. Reproducibility after spliced leader priming without amplification (sample T1 versus sample T2). B. Reproducibility after spliced leader priming without amplification (sample T3 versus sample T4). C. Reproducibility after spliced leader priming and amplification, using trypanosome RNA mixed with a 1000-fold excess of HeLa RNA (sample TH1 versus sample TH2). D. Reproducibility after spliced leader priming and amplification (sample TH3 versus sample TH4). E. Amplification affects read counts with a loss of some genes. The mean read counts for all unamplified samples (T1–T4) are plotted against those for all TH samples (TH1–TH4).