Table 1. The top five RefSeq RNAs that are exclusively identified by FANSe2 in A549 mRNA-seq dataset.
| FANSe2 | RT-PCR validation | |||||
| Gene name | RefSeq-ID | Read count | rpkM | Whole Transcriptome qPCR Primer Database ID | Expected product size (bp) | Validated (Figure 5) |
| PCDHGB3 | NM_018924 | 105 | 1.47 | PCDHGB3_uc003ljw.2_1_2_2 | 111 | Yes |
| SPIN2A | NM_019003 | 60 | 2.92 | PB * | 103 | Yes |
| LOC647859 | NR_026578 | 52 | 5.77 | OCLN_uc011cru.1_2_1_2 | 78 | Yes |
| PNMA6A | NM_032882 | 45 | 1.95 | PB * | 74 | Yes |
| PPIAL4F | NM_001164262 | 44 | 3.60 | PB * | 181 | Yes |
*PB: primer pair not available in whole transcriptome qPCR primer database. The primers are automatically designed using NCBI-PrimerBLAST. Please refer to Table S2 in File S1 for details.