Table 2. Sensitivity and resistance of different mutation sites in HIV-1 CRF_BC pol region to NNRTIs using an in vitro phenotypic assay^a.

Mutations	TMC-125		DLV		NVP		EFV
	EC50a (nM)	Fold changec	EC50 (µM)	Fold change	EC50 (µM)	Fold change	EC50 (nM)	Fold change
WTb (BC)	1.03±0.10	-	0.09±0.00	-	0.10±0.01	-	1.713±0.180	-
W88C (BC)	1.09±0.02	1.06	0.05±0.00	0.51	0.09±0.01	0.89	0.98±0.06	0.57
K101Q (BC)	3.61±0.35	3.50	0.15±0.01	1.71	1.30±0.14	12.57	5.25±1.80	3.06
K103N (BC)	1.400±0.06	1.35	7.35±0.23	82.56	17.23±1.36	167.23	97.48±4.06	56.91
I132L (BC)	5.21±0.78	2.55	1.72±0.12	19.35	4.30±0.26	28.05	10.52±1.22	6.13
R135L (BC)	1.36±0.15	1.32	0.07±0.00	0.75	0.12±0.00	1.13	1.87±0.13	1.09
T139R (BC)	3.73±0.62	1.82	0.42±0.02	4.69	2.59±0.53	25.12	3.23±0.38	1.89
T139K (BC)	4.82±0.96	4.67	0.33±0.02	3.66	0.76±0.02	7.35	5.65±0.12	3.30
M184V (BC)	0.69±0.04	0.67	0.03±0.00	0.31	0.05±0.01	0.44	0.70±0.05	0.41
Y181C (BC)	5.91±1.700	5.73	4.53±0.13	50.92	15.84±1.42	153.74	3.22±0.14	1.88
H221Y (BC)	1.42±0.16	1.38	0.18±0.01	2.04	0.21±0.03	2.02	3.02±0.51	1.76
L228R (BC)	1.02±0.101	0.99	0.042±0.001	0.47	0.11±0.00	1.03	0.79±0.04	0.46
WTb (B)	1.66±0.35	-	0.11±0.00	-	0.19±0.01	-	3.26±0.25	-
I132L (B)	6.51±1.19	3.91	0.51±0.02	4.61	1.20±0.03	6.38	11.61±0.19	3.56
T139K (B)	5.21±1.03	3.13	0.200±0.01	1.78	0.43±0.03	2.26	6.87±0.79	2.10
T139R (B)	9.64±0.87	5.79	0.44±0.01	3.99	1.09±0.05	5.78	8.97±0.63	2.75

Note:

^aThe unit for the EC50 values of TMC-125 and EFV is nM, while that for the EC50 values of DLV and NVP is µM. Data were presented as the mean ± standard deviations of three separate determinations.

^bWT: HIV-1 CRF_BC strain N-14-1.

^cFold change was determined by calculating the ratio of EC50s for mutations and WT viruses.