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. 2014 Apr 17;9(4):e95236. doi: 10.1371/journal.pone.0095236

Figure 4. CAGs-rtTA3 and CAGs-RIK enable GFP induction in myeloid and T lymphocyte lineages.

Figure 4

A. Scatter plots representing GFP and mKate2 expression in Gr1 positive cells in the bone marrow of double transgenic (rtTA/TGM) animals following 1 week of doxycycline treatment (625 mg/kg in chow). B. Quantitation of GFP and mKate2 positive cells in Gr1, Thy1 and CD19 positive populations from the bone marrow, thymus and spleen respectively. Bars represent the mean percentage of GFP or mKate2 positive cells in each tissue, in 3 independent animals (per genotype) +/− SEM. C. Western blot of lysates from control (c57Bl/6), GFP negative and GFP positive splenocytes, indicating rtTA3, GFP and mKate2 expression in each population. Retrovirally transduced 3T3 cells serve as the positive control for rtTA3 expression. D. Graphs represent mRNA abundance in control (C57Bl/6), GFP negative and GFP positive splenocytes.