Figure 3. DD-TCOF1 retains the nucleolar localization of wild-type TCOF1.
Knock-in cells in Shield-1 or ethanol vehicle alone and parental wild-type 293T cells were stained and imaged as described in Materials and Methods. A single representative z-slice confocal image is shown. The visible grayscale cell boundaries represent F-actin (stained with phalloidin).