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. 2014 Apr 17;9(4):e95168. doi: 10.1371/journal.pone.0095168

Figure 5. Time course of osteocalcin production from hPDLCs exposed to LIPUS.

Figure 5

The cells were treated with or without LIPUS and osteocalcin levels in the conditioned media were determined by radioimmunoassay at 5, 7, 9, 11, 13, and 15 days of culture. The release of osteocalcin into the media was continuously increased from day 5 and the production of osteocalcin was significantly higher in the LIPUS stimulation group than that in the control group at each time point. The data are presented as the mean±SD of three separate experiments. * P<0.05 vs. the control group.