Figure 4. Validation of microarray chip data using quantitative RT-PCR.
A The expression of upregulated genes. B The expression of downregulated genes. Results are an average of three biological replicates ± standard error. Genes were normalized using actin and tubulin as housekeeping genes. Genes expression in DEC on the first day of culture were used as control. Statistically significant differences are indicated (* P<0.05 and ** P<0.01 by two-tailed t-test and one-way ANOVA).