Table 2. False negatives for each of the three detection methods compared in this study.
False negatives (Hepatozoon/Eimeriorina) | |||
Microscopy | PCR | qPCR (BK) | |
Microscopy (n = 72) | - | 5/2 | 19/1 |
PCR (n = 87) | 0/2 | - | 16/3 |
qPCR (BK) (n = 87) | 0/0 | 1†/1 | - |
Ct was higher than 35 and differed by more than 1 Ct in two trials so was considered negative (see Materials and Methods).