Table 2. Univariate analysis and multivariate Cox analysis of CD8⁺ CTLs infiltrate status for disease-free survival (DFS) in the entire cohort (n = 332).

Clinicopathological variables	HR	95%-CI	P
A. Univariate analysis
CD8+ CTLs infiltrate status (− vs. +)	0.454	0.265–0.777	0.004
Age (<50 years vs. ≥50 years)	0.987	0.627–1.553	0.954
pT stage (≤2 cm vs. >2 cm)	1.297	0.855–1.969	0.221
Histological grade (G I and II vs. G III)	2.972	1.946–4.538	<0.001
ER status (− vs. +)	0.677	0.436–1.054	0.084
PR status (− vs. +)	0.786	0.512–1.207	0.271
HER-2 status (− vs. +)	1.547	0.900–2.657	0.114
Ki-67 expressiona (Low vs. High)	1.931	1.261–2.957	0.002
B. Multivariate Cox analysis
CD8+ CTLs infiltrate status (− vs. +)	0.418	0.242–0.724	0.002
Age (<50 years vs. ≥50 years)	1.016	0.639–1.618	0.945
pT stage (≤2 cm vs. >2 cm)	1.018	0.657–1.577	0.936
Histological grade (G I and II vs. G III)	3.164	1.965–5.094	<0.001
ER status (− vs. +)	0.776	0.398–1.515	0.458
PR status (− vs. +)	1.447	0.742–2.820	0.278
HER-2 status (− vs. +)	1.256	0.714–2.213	0.429

CTLs cytotoxic T lymphocytes; ER, estrogen receptor; PR progesterone receptor; HER-2 human epidermal growth factor receptor 2; HR hazard ratio; 95%-CI 95%-confidence interval.

^aThe total number of available cases for Ki-67 expression in univariate analysis is 320.