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. 2013 Dec 20;33(1):21–34. doi: 10.1002/embj.201385651

Figure 6.

Figure 6

  1. Derivatives of the maxCFLuc construct were created that contain runs of either GAA or GAG codons, both encoding glutamic acid, following the start codon.
  2. The GAG codon run significantly reduces luciferase activity compared to the GAA run (black bars, n = 16, P < 10–12, t-test) while affecting mRNA levels less significantly (light bars, n = 3, P = 0.12, t-test). Error bars indicate the standard error of the mean. Samples significantly different from GAA are labelled: ***, P < 0.001; unlabelled, P > 0.05.
  3. A summary of parameters for the two Glu codon run constructs. There is a good quantitative match between the observed expression levels differences and the predicted codon decoding time, corroborating a model in which different codons are decoded with different speeds in vivo.