Figure 6.
Local PEPR activation confers systemic immunity.
A–B qRT-PCR analysis for PDF1.2a and PR1 in systemic leaves of 4-week-old plants upon 1 μM Pep2 application for 24 h.
C qRT-PCR analysis for PROPEP2,PROPEP3, and PDF1.2a in systemic leaves upon local Pep2 application as performed in (A) and (B).
D Systemic, non-treated leaves of 4-week-old plants pretreated with a mixture of Pep2 and Pep3 (1 μM each) for 24 h, were spray-inoculated with Ch path-29 (5 × 105 spores/ml). Fungal biomass was determined by qRT-PCR analysis at 5 dpi.
Data information: Bars represent means and SE of at least three biological replicates calculated by the mixed linear model. On the vertical axis, the log2 expression levels relative to that of At4g26410 are shown. Asterisks indicate significant differences from mock-treated WT plants (A, B right, C, and D) or from Pep-treated WT plants (B left) (*q < 0.05, **q < 0.001, two-tailed t-tests).