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. 2014 Apr 17;9(4):e95509. doi: 10.1371/journal.pone.0095509

Figure 1. Representative results of DACH1 expression and methylation in esophageal cancer cells.

Figure 1

(A) DACH1 expression level detected by RT-PCR in esophageal cancer cell lines. (B) Methylation status in promoter region; IVD: in vitro methylated DNA, used as methylation control; NL: normal blood lymphocyte DNA, used as unmethylation control; U: unmethylated alleles; M: methylated alleles. (C) BSSQ of DACH1 promoter region (−426 bp to −140 bp) in KYSE150, KYSE510, TE8 and KYSE140 cells; double-headed arrow: MSP PCR product, spanning 130 bp. Filled circles: methylated CpG sites; open circles: unmethylated CpG sites.