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. 2014 Apr 17;9(4):e95509. doi: 10.1371/journal.pone.0095509

Figure 5. Effect of DACH1 on TGF-β signaling in human esophageal cancer cells.

Figure 5

(A) Smad-binding elements (SBE)-4 Luc reporter activities in KYSE510 and KYSE150 cells. Columns, mean of three independent experiments; bars, SEM. (B) The expression level of TGF-β signaling downstream genes in DACH1 expressed cells and unexpressed cells, β-Actin was used as a loading control. (C) The efficiency of siRNAs targeting on DACH1 in KYSE140 cells. (D) The expression level of TGF-β signaling downstream genes in DACH1-siRNA KYSE140 cells and control group, β-Actin was used as a loading control.