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. 2014 Apr 17;9(4):e95642. doi: 10.1371/journal.pone.0095642

Figure 7. Analysis of seed germination and root elongation of GhMPK17 overexpression transgenic Arabidopsis under mannitol treatment.

Figure 7

(A to C) Seed germination rate of GhMPK17 overexpression lines and wild type on MS medium containing 0 (A), 100 (B) and 200 (C) mM mannitol. Germination rate (root emergence) was evaluated at indicated time after sowing. (D to G) Phenotype of wild type and GhMPK17 overexpression seedlings under mannitol treatment. Arabidopsis seeds were germinated on MS medium under normal condition for 7 days and then the seedlings were transferred onto MS medium supplemented with 0 (D), 100 (E), 200 (F) and 300 (G) mM mannitol for 7 days. Bars = 1 cm. (H to K) Average root elongation histogram of wild type and GhMPK17 overexpression transgenic lines grown on MS medium (H), or MS medium supplemented 100 (I), 200 (J) and 300 (K) mM mannitol, respectively. (L and M) Statistical analysis on the quantity and length of the lateral roots of GhMPK17 transgenic lines and wild type on MS medium (L) or supplemented 100 mM mannitol (M). The lateral roots of GhMPK17 transgenic lines and wild type were divided into several groups according to their length (mm) and the quantity that each group occupied is shown. (N and O) Histogram of lateral roots quantity of wild type and GhMPK17 overexpression lines on MS medium (N) or supplemented 100 mM mannitol (O). Data were shown from three independent experiments, and bars indicate SDs. *, P<0.05 and **, P<0.01. WT, wild type; L11, L24 and L46, GhMPK17 transgenic line 11, 24 and 46.