Figure 2. Comparison of the CO₂ assimilation rate as a function of C _c, modelled with the kinetic parameters of Rubisco in coffee and several C₃ species.

The RuBP saturated rates of CO₂ assimilation at 25°C were calculated using the following equation [13]: . C _c, the chloroplastic CO₂ partial pressure; O, the intercellular O₂ partial pressure; Γ*, the CO₂ compensation point in the absence of day respiration; [Rubisco], the catalytic site content of Rubisco. The Rubisco kinetic properties for coffee (Table 3) and the kinetic properties for the other species were retrieved from Savir et al. [69] and are summarized in Table S1. The R _L and [Rubisco] were set as 1.0 µmol CO₂ m⁻² s⁻¹ and 25 µmol sites m⁻², respectively.