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. 2014 Apr 17;10(4):e1004051. doi: 10.1371/journal.ppat.1004051

Figure 6. AtRH2 and AtRH5 promote plus-strand synthesis on partial dsRNA templates by the affinity-purified tombusvirus replicase.

Figure 6

(A) Scheme of the partial dsRNA templates used. Note the presence or absence of RIII(−) REN in the templates. (B) Representative denaturing gel of 32P-labeled RNA products synthesized by the purified tombusvirus replicases obtained from yeast with depleted Fal1p is shown. The level of complementary RNA synthesis on the partial dsRNA templates was compared in the presence of purified recombinant AtRH2 (0.4 µg), AtRH5 (0.4 µg) and AtRH20 (1.0 µg) helicases. Note that this replicase preparation produces mostly (+)RNA products when using dsRNA templates. Each experiment was repeated three times.