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. 2014 Apr 17;9(4):e95615. doi: 10.1371/journal.pone.0095615

Figure 1. Generation of the RyR2 Ex3-del mutant mouse model.

Figure 1

(A) The mouse ryr2 locus (top line), the construction of the knock-in (KI) vector containing the deletion of the exon-3 and flanking intron sequences in the mouse ryr2 gene, the selectable markers neo (neomycin resistant gene) and DTA (diphtheria toxin A), the FRT sites (middle line), the generation of the exon-3 deletion mutant allele, and the removal of the neo gene via homologous recombination (bottom line) are depicted. (B) Intron and exon sequences that were deleted in the RyR2 Ex3-del mutant mice. (C) Southern blot analysis of recombinant embryonic stem cells (WT, wild type; Het, heterozygous). (D) PCR genotyping using tail samples from WT and Ex3-del heterozygous (Het) mice.