Figure 2. qPCR validation performed on selected genomic regions.

(A) Results for the region 11q11. (B) Results for the region 13q12.11. A copy number of around 2 was deemed to be indicative of wild-type status (i.e. no CNV), a copy number of 1 was indicative of one copy lost, whereas a copy number of 3 or above was held to indicate copy number gain(s). The error bars represent the standard error among four replicates.