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. 2014 Apr 17;9(4):e95625. doi: 10.1371/journal.pone.0095625

Figure 3. Effect of DFS-ICSI on the tail kinking phenotype of the offspring.

Figure 3

Tail phenotypes (1: none or slightly kinky; 2: medium kinky; 3: very kinky) recorded in all the Axin1F/+ offspring in the groups: in vivo-produced controls, oocytes fertilized in vivo, cultured for 24 h and transferred at the 2-cell stage, and DFS-ICSI-fertilized oocytes transferred at the 2-cell stage. DFS-ICSI led to a kinkier tail phenotype. Error bars represent mean ± SD. Bars with different lowercase letters (a, b, c) represent significant differences for each phenotype (P≤0.01).