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. 2014 Apr 17;10(4):e1004074. doi: 10.1371/journal.ppat.1004074

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© 2014 Katris et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Constitutive secretion of microneme proteins MIC2 (A, B) and AMA1 (D, E) from extracellular iΔHA-RNG2 cells grown with or without ATc. Secretion was also assessed with cGMP stimulation by Zaprinast (A, D), or with calcium stimulation by A23187 (B, E). Tom40 detection in cell pellets provides a control for parasite number used for secretion assays. (C, F) Secretion averages from biological replicates (n = 6 for constitutive (Con.) secretion; n = 4 for stimulated secretion). Error bars = standard error of the mean.