B6 calvarial osteoblasts differentiated for 14 and 21days, OCR (a and c) and ECAR (b and d) profiles of B6 calvarial osteoblasts that have undergone differentiation for 14 days (a and b) and 21 days (c and d). The data shown are representative of 2 individual runs for the 14-day assay and an average of 2 independent runs for the 21-day assay. (e, i, ii, iii) Bar graphs for OCR:ECAR ratio for B6 calvarial osteoblasts that have been differentiated for 7, 14, and 21 days, respectively, in osteogenic differentiation media and compared with nondifferentiation media. Diff, differentiation; Non Diff, nondifferentiation.