Figure 2.

ADP inhibits GnRH neuronal activity. A, Calcium Green-1 imaging of GnRH neurons. Cells were identified by their bipolar morphology (A₁, bright field), loaded with fluorescent calcium-sensitive dye (A₂, fluorescence), and their identity was verified after imaging by ICC (A₃, immunofluorescence). Arrows indicate identical cells in all fields. B, Representative recording showing spontaneous baseline oscillations in intracellular calcium levels in a single GnRH neuron during 30 minutes in SFMs (Y-scale, OD units). C, Representative recording showing spontaneous baseline oscillations in intracellular calcium levels in a GnRH neuron during 10 minutes in SFM and subsequent decrease in activity during a 10-minute superfusion of ADP (500 ng/mL) (Y-scale, OD units). D, Concentration of ADP (0.1–5 μg/mL) vs the percent of GnRH cells that were inhibited.