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. 2014 Feb 24;155(5):1851–1863. doi: 10.1210/en.2013-1677

Figure 5.

Figure 5.

ADP inhibits GnRH neurons via activation of AdipoR2 and subsequent PCK/LKB1 activation of AMPK. A, Schematic representation of AdipoR pathways that could possibly elicit the inhibitory effect of ADP. B, PCR on single GnRH cell cDNAs revealed expression of PKCζ and LKB1. GnRH neurons were immunopositive for PKCζ (C) and LKB1 (D). E, Coapplication of PPS (10μM, PKCζ pseudosubstrate, a PKCζ antagonist) together with ADP (500 ng/mL) prevented the inhibitory effect of ADP on the frequency of calcium oscillations in GnRH neurons. A representative calcium recording is shown. F, Coapplication of PPS (10μM) together with AICAR (500μM) did not prevent the inhibitory effect of AICAR on the frequency of calcium oscillations in GnRH neurons. A representative calcium recording is shown. Scale bar, 10 μm (C and D).