Figure 6.
Tissue-specific maintenance of IVFKAA-induced changes to Txnip regulation in 30-week-old mice. A, Txnip mRNA expression relative to H2A in whole pancreas/pancreatic islets, liver, muscle, and fat tissues for female (spotted) and male (hatched) FB (blue) and IVFKAA (yellow) animals. B, Western blot of TXNIP and β-actin expression in female muscle and fat, and male fat tissue, with corresponding quantification of expression. C and D, Chromatin immunoprecipitation at the Txnip and Gapdh promoters in male and female fat tissue for (C) H4ac and (D) H4K20me3 enrichment in FB and IVFKAA adults. E, H4ac enrichment in female liver at Txnip and Gapdh promoters. Error bars depict SD. *, P < .05.