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NMDA-induced GnRH neuron activation. A, Representative photos for c-Fos-ir (black arrowhead) and negative (white arrowhead) in GnRH neurons (scale bar, 10 μm, magnification, 40×). B, Number of GnRH-ir cells. C, Number of c-Fos-ir cells. D, Percentage of GnRH neurons coexpressing c-Fos. Values are shown as means ± SEM (n = 3). a, P < .0001 vs all Y-aCSF; b, P < .001 vs 15 mg/kg Y-aCSF; c, P < .001 vs 15 mg/kg Y-JB1+E2, MA-aCSF+E2, and MA-IGF-I+E2.
