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. 2014 Mar 10;155(5):1827–1837. doi: 10.1210/en.2013-1682

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Figure 5. — Effects of chronic IGF-I or JB-1 infusion in E2- or oil-injected young (Y) and middle-aged (MA) females on mRNA expression in the posterior hypothalamus. esr1 in Y (A) and MA (B) females; IGF-Ir in Y (C) and MA (D) females; kiss1r in Y (E) and MA (F) females; nr1 in Y (G) and MA (H) females; and nr2b in Y (I) and MA (J) females. β-Actin was used as the housekeeping gene for transcript normalization. Values are shown as means ± SEM (n = 4). a, P < .05 vs Y-aCSF-oil; b, P < .05 vs Y-aCSF-E2; c, P < .05 vs MA-aCSF-oil and MA-aCSF-E2. Veh, vehicle.