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The only clinically used test that detects HPV DNA, in situ hybridization (ISH), utilizes oligonucleotide probes specific for the DNA of several high-risk HPV types (A, 40X magnification). Also shown is a slide stained as negative control for ISH (B). Immunohistochemistry for p16 (A, 4X magnification & B, 40X magnification) is another commonly accepted test to assess for HPV status with p16-positive tumors being deemed HPV-positive.
