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| Component | Final concentration | Stock | Amount |
|---|---|---|---|
| Tris–HCl (pH 7.6) | 330 mM | 1 M | 1.65 ml |
| MgCl2 | 50 mM | 1 M | 250 μl |
| Dithiothreitol | 5 mM | 1 M | 25 μl |
| ATP | 5 mM | 100 mM | 250 μl |
| Polyethylene glycol (PEG 6000) | 30% | 100% | 1.5 ml |
Add water to 5 ml
Note: This buffer can be purchased from New England Biolabs as part of the NEBNext Master Mix 3 (cat# E6060S/L) or as an individual buffer (cat# E6062A/AA)
