End repaired DNA from Step 2 | Variable |
---|---|
5× NEBNext Quick Ligation Reaction Buffer | 40 μl |
P1 DNA adaptor (50 μM) | Variable |
P2 DNA adaptor (50 μM) | Variable |
T4 DNA ligase | 10 μl |
Sterile water | to 200 μl |
End repaired DNA from Step 2 | Variable |
---|---|
5× NEBNext Quick Ligation Reaction Buffer | 40 μl |
P1 DNA adaptor (50 μM) | Variable |
P2 DNA adaptor (50 μM) | Variable |
T4 DNA ligase | 10 μl |
Sterile water | to 200 μl |