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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: J Immunol. 2014 Feb 28;192(7):3390–3398. doi: 10.4049/jimmunol.1302525

Figure 4.

Figure 4

Confirmation of LARG KD. HMVECs were treated with control or LARG shRNA lenti-virus for 48 h and selected for with 2.5 ng/ml puromycin for 24 h. (A) EC lysates were immunoblotted with the indicated pAb. (B) Western blotting shows that ICAM-1 expression before and after TNF-treatment is not affected by LARG KD. (C) Electrical impedance was used to measure monolayer integrity for HMVECs plated at high density for 72 h. No significant difference was found in impedance values after control or LARG KD. n=3 independent experiments preformed in triplicate wells.