Figure 7.

Effect of DLP1 blockade accomplished by introduction of DLP1^K38A or siRNA-DLP1 to AD cybrid cells. A–D) AD cybrid cells were transfected with GFP-DLP1^K38A or empty vector. After 24 h, cells were incubated with Mitotracker Red to analyze mitochondrial morphology under confocal microscopy (Scale bar = 5 µm). DLP1^K38A-transfected AD cybrids had tubular mitochondria, whereas empty vector-transfected cells retained fragmented mitochondrial morphology (A). Mitochondrial average length (B), density (C), and CcO activity (D) were increased in DLP1^K38A-transfected cells compared to vector-transfected control cells. *p<0.05 versus vector treated group. E–J) Effect of siRNA-DLP1. Immunoblotting for DLP1 in cells treated with siRNA-DLP1 (siRNA) or control siRNA (con) (E). Representative immunoblots for DLP1 and β-actin. (F) Mitotracker staining and immunostaining of DLP1 in siRNA-DLP1 or control siRNA treated cells (Scale bar = 5 µm). Average mitochondrial length (G) and density (H).I–J), increased TMRM intensity (I), and ATP levels (J) in siRNA-DLP1 cells relative to control siRNA cells. *p<0.05 versus vector treated group. N = 5–7 cell lines/group.