Figure 3.

Sites of Rrp5 Association with ITS1

(A) Mapped reads for Rrp5-HTP full-length in vitro (purple) and in vivo (gray).

(B) Mapped reads for Rrp5NT-HTP (red) and Rrp5CT-HT (blue) in vivo. The graphs show hit density for each nucleotide over the ITS1 region, per 10,000 total reads mapped within ITS. Processing sites A₂ and A₃ are indicated on the graphs, as well as the major peaks annotated as c and d.

(C) Predicted secondary structure of the yeast ITS1 region. The binding sites identified in vitro for full-length Rrp5-HTP (purple) and in vivo for Rrp5NTD-HTP (red) or Rrp5CTD-HTP (blue) are indicated. Mutated nucleotides are designated by dots alongside the sequence, using the same colors.