Figure 6.

Lytic Water Coordination in the Catalytic Pockets of mkTIP49 Hexamers

(A and B) An overall view of the pocket (A) and the zoom on the γ-phosphate region (B) are shown with ATP (yellow), Mg²⁺, and a water molecule in the attacking position.

(C) Lytic water in the attacking position in the wild-type mkTIP49 protein (left) and in the N326Q variant (right). The N- to Q substitution in the Sensor I motif of mkTIP49 may result in reorientation of water molecules from lytic to non-lytic positions by the mutated Q326 residue and interfere with coordination of Mg²⁺ by D296 of Walker B.