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. 2014 Apr 10;175(100):38–44. doi: 10.1016/j.jbiotec.2014.01.032

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© 2014 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).

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Fig. 2 — Selection and characterization of stable miRNA expression clones: miRNA overexpression was driven by a constitutive CMV promoter with co-expression of GFP. (A) The panel shows GFP-expression (Fl-1H) of 4 stable-pools overexpressing the miR-17–92 cluster (SCM-1792), miR-17 (SCM-17), miR-92a (SCM-92a) and the no-miR-vector control (NC) after the entire selection process. All graphs are overlaid with fluorescence of untransfected cells. (B) qPCR was performed to assess the miRNA overexpression. The expression of miR-17 and miR-92a relative to miR-185 expression (endogenous control) was determined using the ΔΔC_t method. Average fold differences were calculated by normalizing the relative expression (ΔC_t values) to that of the negative control transfection.