Figure 4.

Phenotypic and Functional Characterization of Macrophages Stimulated with TNF, PGE₂, and P3C

(A) Flow cytometry of CD14, CD23, CD25, CD86, CXCR7, CD197, in M^b (dark gray), IFN-γ (light blue), IL-4 (red), and TPP (light gray) activated macrophages. Mean fluorescence intensities (MFI) of at least three independent experiments are shown (mean and SEM; p^∗ < 0.05 Student’s t test).

(B) Immunoblot analysis of STAT4 and β-actin. STAT4 expression was normalized to β-actin expression and set to 100% in M^TPP (TPP).

(C) CXCL5 and IL1α in supernatants of macrophage cultures.

(D) T cell activation by CD3 and CD28 beads in presence or absence of macrophages assessed by CFSE dilution.

(E) Heatmap showing fold changes of highly abundant miRNAs up- or downregulated (FC > 2, FDR adjusted p value < 0.05) in M1 (IFN-γ) or M2 (IL-4), or M^TPP (TPP) compared to M^b (baseline). Fold changes colored from blue to red.

For Figures 4A–4D, mean ± SEM (p^∗ < 0.05, Student’s t-test) are shown.