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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: J Pharm Biomed Anal. 2013 Apr 16;83:1–9. doi: 10.1016/j.jpba.2013.04.007

Figure 1. Connections of the Sample Card and Preparation (SCAP) autosampler, switching valves, HPLC equipment, and mass spectrometer for automated DBS analysis.

Figure 1

(A) Loading of internal standard D4 acetaminophen into the loop. (B) extraction of DBS and LC-MS/MS analysis, (C) system wash and backflush of pre-column and analytical columns.